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Yes, travelers entering the Czech republic by bus must complete and submit the Passenger Locator Form. This Czech government webpage shows the form, which says:
PUBLIC HEALTH PASSENGER LOCATOR FORM RULES FOR ENTERING THE CZECH REPUBLIC
All passengers travelling to the Czech Republic have to fill the Public Health Passenger Locator Form before entering the country.
1 Online form is available on the website http://www.prijezdovyformular.cz
2 After a completed form is sent, you will receive a confirmation email.
3 You have to submit the comfirmation email before bo[a]rding a plane or a bus and during the border and residence control.