Is there any public transport from Punta Arenas, Chile to El Calafate, Argentina?

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Yes there are. There are several bus lines that I could find online though I could only find 1 that provides cross border routes:

If you look at their schedule you will find that they have a bus from Puerto Natales to El Calafate and a connecting bus from Punta Arenas to Puerto Natales though I can't find the trip duration so it is possible that you may have to spend the night at Puerto Natales.

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