Upvote:3
It looks like your biometric validity is only as valid as long as your DS160. Since your application was rejected, it's no longer valid.
In either case, that's the very least of your problems. Unless there was an error on the part of the consulate in your first application processing or your circumstances changed, your re-application is likely to be denied again.
I would worry much more about being denied than about whether you'll be fingerprinted again.