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About the time of Ambrose, Gregory of Nyssa wrote the following in which he speculates about her virginity.
Thus, Miriam’s timbrel being a dead thing, and virginity being a deadening of the bodily passions, it is perhaps not very far removed from the bounds of probability that Miriam was a virgin. However, we can but guess and surmise, we cannot clearly prove, that this was so, and that Miriam the prophetess led a dance of virgins, even though many of the learned have affirmed distinctly that she was unmarried, from the fact that the history makes no mention either of her marriage or of her being a mother; and surely she would have been named and known, not as “the sister of Aaron1477,” but from her husband, if she had had one; since the head of the woman is not the brother but the husband. Gregory of Nyssa
His reference to "many of the learned" and who they are may only be speculated, but clearly by his time, there was teaching that Miriam was a virgin that the tad bit later Chromatius had picked up.
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St. Ambrose wrote in his De virginibus (377 A.D.) bk. 1 ch. 3 (or pp. 202-203 of the Latin):
"So Mary [Miriam] the prophetess, the sister of Aaron, took a timbrel in her hand: and all the women went forth after her with timbrels and with dances" (Exodus 15:20). But consider whom she was then representing. Was she not a type of the Church, who as a virgin with unstained spirit joins together the religious gatherings of the people to sing divine songs? For we read that there were virgins appointed also in the temple at Jerusalem. But what says the Apostle? "Now all these things happened to them in figure: and they are written for our correction, upon whom the ends of the world are come" (1 Corinthians 10:11). For the figure is shown in few, the life exists in many.
Chromatius's Tractatus in Matthaeum comes a bit later, after circa 392-398 according to Duval in «Chromace et Jérôme», as St. Jerome was silent on it.